BIOL 622L - Biological Electron Microscopy

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Theory and practice of the study of biological materials with the high resolution of electron microscope. Specimens will be prepared for examination by a variety of modern procedures. These include tissue and cell fixation, embedding for ultra-thin sectioning, carbon film preparation, mounting of particulate materials and macromolecules, positive and negative staining, metal shadowing in the vacuum evaporator and critical point drying. An introduction to scanning electron microscopy will be provided. The photographic darkroom procedures required for the production of finished electron micrographs are included. Prerequisite: BIOL 303 and/or the consent of instructor.