BIOL 635L - Advanced Molecular Biology Laboratory

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This two-semester course is designed to demonstrate the approaches and methodologies of molecular biology used to study the organization, expression and regulation of genes. Emphasis is on acquiring facility in the design of experiments, analysis of data and troubleshooting, as well as on developing a broad range of technical skills and the ability to use modern laboratory equipment. The first part of the course focuses on in vitro or recombinant DNA/cloning techniques and emphasizes the isolation, manipulation and molecular characterization of DNA and RNA. The second part involves in vivo genetic analysis in selected prokaryotic and eukaryotic organisms. It includes mutagenesis and genetic mapping in E.coli, as well as use of gene fusions to assess gene expression in vivo. Eukaryotic cell culture and immunological techniques are used to illustrate their utility in molecular biology. Note: Enrollment priority will be given to AMB students. Prerequisite: BIOL 624, CHEM 437 and CHEM 437L. Recommended: CHEM 438.